摘要

柠檬酸浸液发酵金属离子浓度的统计设计与优化

作者(年代):阿南德·基肖尔和g·文卡特·雷迪

本文报道了黑曲霉NCIM 705沉水发酵柠檬酸过程中金属离子的统计设计和优化。柠檬酸是通过发酵合成的大宗化学品之一。天然柠檬酸的供应是有限的，因此可以通过发酵过程来满足需求。近年来，黑曲霉主要用于大规模生产柠檬酸。发酵条件的优化对发酵过程的经济性有很大的影响。研究发酵过程中不同物理和化学参数之间的相互作用，可以对发酵过程进行满意的建模和优化。铜(Cu2+)、锌(Zn2+)和镁(Mg2+)被认为是黑曲霉生物合成柠檬酸的重要金属离子。采用响应面法(RSM)中的中心复合设计(CCD)方法，研究了CuSO4.5H2O、ZnSO4.7H2O和MgSO4.5H2O三种离子对柠檬酸产率的影响。采用统计Design-Expert软件建立回归模型，预测柠檬酸离子产率的综合效应。对实验数据拟合二阶多项式方程，采用多元回归法建立响应面模型。 The response of ions was predicted by the polynomial equation. With the citric acid production values computed using polynomial equation, the significance of effect of each ion was analyzed using Analysis of variance (ANOVA). It was observed that copper and zinc ions showed good effect on citric acid production whereas magnesium had no impact. With the citric acid production values predicted by CCD second order equation, three-dimensional response surface curves were obtained using RSM. From the studies, 59.592 mg/L of maximum citric acid was fiund to be produced at the optimum ion concentrations of copper: 1.47 mg/L, zinc: 2.46 mg/L and magnesium : 1.25 mg/L.