文摘

同时测定甲硝唑和呋喃唑酮结合平板剂型:开发和验证稳定性指示高效液相色谱方法

作者(年代):Murali D和Rambabu C

一个敏感、准确、准确与紫外线stability-indicating高效液相色谱检测方法已经开发了同时测定甲硝哒唑、呋喃唑酮。色谱分离是实现Purospher®明星RP-18列(250毫米×4.6毫米;5μm粒度)的流动相由乙腈和水(挺,v / v)和0.8毫升/分钟的流量。检测波长被设定为332海里。甲硝唑和呋喃唑酮受到不同的强制降解条件。在所有的情况下,降解产物是解决从甲硝哒唑、呋喃唑酮的山峰。该方法线性浓度范围为30μg /毫升到90μg /毫升(R2 = 0.9999)和10μg /毫升30μg /毫升(R2 = 0.9996)甲硝哒唑、呋喃唑酮,分别。定量的极限是0.793μg /毫升和0.230μg /毫升甲硝唑和呋喃唑酮,分别。方法的精度是令人满意的;相对标准偏差值不超过1%。 The accuracy of the method was proved; the mean recovery of metronidazole and furazolidone was in the range of 99.66% to 100.28%. The developed and validated method was applied successfully for the assay of metronidazole and furazolidone in combined tablet dosage with good precision and accuracy.