摘要
，卷:10(2)DOI: 10.37532/2277-288X.2020.10(1).141
�??PAICO�?注射液消化作用机制的研究�(羊绒藜)
在阿根廷，民族医学已经有500多年的历史。迄今为止，只有少量具有药用活性成分的蔬菜被研究过。本研究回顾了一种由来自阿根廷圣克鲁斯戈伯纳多尔·格雷戈尔斯地区(48.75 S - 70.24 W)的蔬菜制成的可能成分和作用机制，当地居民称之为“paico”(Chenopodium ambrosioides)。所述输液是利用蔬菜的空中部分，主要用作消化。这项试验的目的是在“体外”发现“paico”(“I.P.”)输液的药理特性。作为生物材料，选用秋季采集的柏树叶和种子，用塔架在砂浆中完全碾碎。所用的水都是蒸馏水。蛋清也被使用。所用试剂为:盐酸(0.1 N HCl)，实验室商业白蛋白(BSA)。实验室的Biorgen和Biuret和酪蛋白试剂。 Francisco Walz. The following tests were carried out in the School Laboratory of the Agricultural School No. 1 in order to estimate the activity of the infusion: 1 - Hydro-distillation of the essential oil. 2 - Biuret reaction. 3 - Action of the (“I.P.”) on the pH of a protein solution. 1-Hydro-distillation of essential oil. The equipment was assembled in a similar way to the Clevenger distiller. The crushed paico leaves and seeds were worked. Careful heating to boiling with adequate reflux began for 90 minutes with the addition of water distilled by the lock to always maintain the original water level. The apparatus was allowed to cool slightly and the water and oil were collected in an Erlenmeyer flask. This procedure was repeated numerous times. 2-Biuret reaction. The Biuret reaction is a characteristic reaction of the protein search. In the procedure of this test, the raw蛋白色在一升冷水中搅拌，并使其沸腾，不搅拌。它泄露。得到的液体是变性白蛋白(白蛋白)的精细悬浮液。另一方面，(“ip”)是准备好的。将5克压碎的柏柯叶和种子放入100毫升水中，煮沸2分钟，然后冷却。让它静置12分钟，然后过滤。在四个试管中制备了以下混合物:试管1。- 6毫升白蛋白+ 6毫升水。管2。- 6毫升白蛋白+ 1.5毫升水+ 4.5毫升盐酸，0.1 n。- 6ml白蛋白+ 1.5 ml(“IP”)+ 4.5 ml HCl1, 0.1 n。 - 6 ml of albumin + 1.5 ml of (“IP”) + 4.5 ml of water. 2 ml of Biuret's reagent was placed in all tubes. Then the tubes were placed in a water bath, at 40 ° C. A few minutes later, the tubes take on a pinkish-purple color, only in Tube 3 did a rinsing occur, this is a consequence of the activity of the (“IP ”) That, in an acidic medium, has hydrolyzed albumin. 3-Action of the (“I.P.”) on the pH of a protein solution. A third test was carried out using commercial albumin and casein as protein sources to which the (“I.P.”) was added. 1 gram solutions of the two protein sources were prepared separately in beakers, to which was added 40 ml of distilled water. 5 ml of the ("I.P.") was placed into each solution thus prepared. In another glass, he placed 1 g casein and 40 ml of distilled water, in the next 1 g of commercial albumin and 40 of distilled water and in the last beaker (“I.P.”). Immediately in all the beakers the pH was continuously measured for 15 minutes. The results of the tests were: in the hydro-distillation of the essential oil a few drops of the essential oil were obtained, approximately 0.5 ml for each test. They are reserved for after several tests and having collected about 10 ml, send to a laboratory with a chromatograph.
摘要
在阿根廷，民族医学已经有500多年的历史。迄今为止，只有少量具有药用活性成分的蔬菜被研究过。本研究回顾了一种由来自阿根廷圣克鲁斯戈伯纳多尔·格雷戈尔斯地区(48.75 S - 70.24 W)的蔬菜制成的可能成分和作用机制，当地居民称之为“paico”(Chenopodium ambrosioides)。所述输液是利用蔬菜的空中部分，主要用作消化。这项试验的目的是在“体外”发现“paico”(“I.P.”)输液的药理特性。作为生物材料，选用秋季采集的柏树叶和种子，用塔架在砂浆中完全碾碎。所用的水都是蒸馏水。蛋清也被使用。所用试剂为:盐酸(0.1 N HCl)，实验室商业白蛋白(BSA)。实验室的Biorgen和Biuret和酪蛋白试剂。 Francisco Walz. The following tests were carried out in the School Laboratory of the Agricultural School No. 1 in order to estimate the activity of the infusion: 1 - Hydro-distillation of the essential oil. 2 - Biuret reaction. 3 - Action of the (“I.P.”) on the pH of a protein solution. 1-Hydro-distillation of essential oil. The equipment was assembled in a similar way to the Clevenger distiller. The crushed paico leaves and seeds were worked. Careful heating to boiling with adequate reflux began for 90 minutes with the addition of water distilled by the lock to always maintain the original water level. The apparatus was allowed to cool slightly and the water and oil were collected in an Erlenmeyer flask. This procedure was repeated numerous times. 2-Biuret reaction. The Biuret reaction is a characteristic reaction of the protein search. In the procedure of this test, the raw蛋白色在一升冷水中搅拌，并使其沸腾，不搅拌。它泄露。得到的液体是变性白蛋白(白蛋白)的精细悬浮液。另一方面，(“ip”)是准备好的。将5克压碎的柏柯叶和种子放入100毫升水中，煮沸2分钟，然后冷却。让它静置12分钟，然后过滤。在四个试管中制备了以下混合物:试管1。- 6毫升白蛋白+ 6毫升水。管2。- 6毫升白蛋白+ 1.5毫升水+ 4.5毫升盐酸，0.1 n。- 6ml白蛋白+ 1.5 ml(“IP”)+ 4.5 ml HCl1, 0.1 n。 - 6 ml of albumin + 1.5 ml of (“IP”) + 4.5 ml of water. 2 ml of Biuret's reagent was placed in all tubes. Then the tubes were placed in a water bath, at 40 ° C. A few minutes later, the tubes take on a pinkish-purple color, only in Tube 3 did a rinsing occur, this is a consequence of the activity of the (“IP ”) That, in an acidic medium, has hydrolyzed albumin. 3-Action of the (“I.P.”) on the pH of a protein solution. A third test was carried out using commercial albumin and casein as protein sources to which the (“I.P.”) was added. 1 gram solutions of the two protein sources were prepared separately in beakers, to which was added 40 ml of distilled water. 5 ml of the ("I.P.") was placed into each solution thus prepared. In another glass, he placed 1 g casein and 40 ml of distilled water, in the next 1 g of commercial albumin and 40 of distilled water and in the last beaker (“I.P.”). Immediately in all the beakers the pH was continuously measured for 15 minutes. The results of the tests were: in the hydro-distillation of the essential oil a few drops of the essential oil were obtained, approximately 0.5 ml for each test. They are reserved for after several tests and having collected about 10 ml, send to a laboratory with a chromatograph.
RA¤umungsfirma维恩RA¤umungsdienst维恩
在阿根廷，民族医学已经有500多年的历史。迄今为止，只有少量具有药用活性成分的蔬菜被研究过。本研究回顾了一种由来自阿根廷圣克鲁斯戈伯纳多尔·格雷戈尔斯地区(48.75 S - 70.24 W)的蔬菜制成的可能成分和作用机制，当地居民称之为“paico”(Chenopodium ambrosioides)。所述输液是利用蔬菜的空中部分，主要用作消化。这项试验的目的是在“体外”发现“paico”(“I.P.”)输液的药理特性。作为生物材料，选用秋季采集的柏树叶和种子，用塔架在砂浆中完全碾碎。所用的水都是蒸馏水。蛋清也被使用。所用试剂为:盐酸(0.1 N HCl)，实验室商业白蛋白(BSA)。实验室的Biorgen和Biuret和酪蛋白试剂。 Francisco Walz. The following tests were carried out in the School Laboratory of the Agricultural School No. 1 in order to estimate the activity of the infusion: 1 - Hydro-distillation of the essential oil. 2 - Biuret reaction. 3 - Action of the (“I.P.”) on the pH of a protein solution. 1-Hydro-distillation of essential oil. The equipment was assembled in a similar way to the Clevenger distiller. The crushed paico leaves and seeds were worked. Careful heating to boiling with adequate reflux began for 90 minutes with the addition of water distilled by the lock to always maintain the original water level. The apparatus was allowed to cool slightly and the water and oil were collected in an Erlenmeyer flask. This procedure was repeated numerous times. 2-Biuret reaction. The Biuret reaction is a characteristic reaction of the protein search. In the procedure of this test, the raw蛋白色在一升冷水中搅拌，并使其沸腾，不搅拌。它泄露。得到的液体是变性白蛋白(白蛋白)的精细悬浮液。另一方面，(“ip”)是准备好的。将5克压碎的柏柯叶和种子放入100毫升水中，煮沸2分钟，然后冷却。让它静置12分钟，然后过滤。在四个试管中制备了以下混合物:试管1。- 6毫升白蛋白+ 6毫升水。管2。- 6毫升白蛋白+ 1.5毫升水+ 4.5毫升盐酸，0.1 n。- 6ml白蛋白+ 1.5 ml(“IP”)+ 4.5 ml HCl1, 0.1 n。 - 6 ml of albumin + 1.5 ml of (“IP”) + 4.5 ml of water. 2 ml of Biuret's reagent was placed in all tubes. Then the tubes were placed in a water bath, at 40 ° C. A few minutes later, the tubes take on a pinkish-purple color, only in Tube 3 did a rinsing occur, this is a consequence of the activity of the (“IP ”) That, in an acidic medium, has hydrolyzed albumin. 3-Action of the (“I.P.”) on the pH of a protein solution. A third test was carried out using commercial albumin and casein as protein sources to which the (“I.P.”) was added. 1 gram solutions of the two protein sources were prepared separately in beakers, to which was added 40 ml of distilled water. 5 ml of the ("I.P.") was placed into each solution thus prepared. In another glass, he placed 1 g casein and 40 ml of distilled water, in the next 1 g of commercial albumin and 40 of distilled water and in the last beaker (“I.P.”). Immediately in all the beakers the pH was continuously measured for 15 minutes. The results of the tests were: in the hydro-distillation of the essential oil a few drops of the essential oil were obtained, approximately 0.5 ml for each test. They are reserved for after several tests and having collected about 10 ml, send to a laboratory with a chromatograph.
