文摘
SOCS3的效果对人类肝癌的上皮间质转化MHCC97-H细胞
作者(年代):礼乐”王研究抑制细胞因子信号的影响3 (SOCS3)上皮间充质转变人类肝癌细胞系MHCC97-H (EMT)。方法:人类肝癌细胞株MHCC97-H在体外培养。细胞被瞬变转染25 nmol / L SOCS3核(阳性转染组)和空质粒转染对照组(负)。24小时后,转染细胞的荧光表达被荧光显微镜观察。48小时后,细胞的形态变化被倒置显微镜观察。钙粘蛋白的表达,EMT的上皮标记,和间质标记a-SMA MHCC97-H细胞被免疫荧光染色检测。结果:与阴性对照组相比,MHCC97-H细胞成功转染SOCS3 siRNA显示绿色荧光。SOCS3 siRNA瞬时转染后,肝癌细胞与上皮样的特征从鹅卵石形状轴和轴形状与间质细胞形态。钙粘蛋白的表达和a-SMA被免疫荧光检测。结果表明,e-eadherin的表达,小干扰rna阳性转染组上皮SOCS3的标志,显著降低,而a-SMA的表达,间质细胞的标志,显著增加。 Conclusion: This study shows that the down-regulation of SOCS3 expression in hepatoma cells can promote the proliferation of hepatoma cells by changing their EMT phenotype, molecular and phenotypic characteristics, suggesting that SOCS3 may play an important role in the EMT of hepatoma cells. Regulating the expression level of SOCS3 can inhibit the occurrence and development of liver cancer, and provide a new idea for clinical prevention and treatment of liver cancer.
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