文摘
在酵母合成和自组装的噬菌体粒子:小说分子工具箱
作者(年代):Aliona Spakova, Raminta Batiuskaite,拉莎Petraityte-Burneikiene, Eugenijus Simoliunas, Lidija Truncaite,维达Casaite和Rolandas Meskys问题的声明:目前传染病主要依靠减毒疫苗或灭活的病原体。然而,病毒像粒子(车牌区域)作为疫苗平台更有利于他们的完美定义的结构;诱导强烈的免疫应答,也适合表面装饰通过插入外国抗原表位。虽然许多二十面体等细菌和缺点的一种合成缺乏真核蛋白质需要转录后修饰和木糖醇的纯化一种污染。二十面体车牌区域平台作了详细研究但杆状种绝大部分都被遗忘了。直到现在,没有信息的生成跟踪噬菌体纳米管在酵母。目的:该研究旨在生成碳纳米管使用酵母表达了噬菌体尾巴蛋白质和决定他们对基因引入外国抗原表位。方法:尾部DNA序列编码蛋白的噬菌体NBD2, FV3以及RaK2被克隆到酵母蛋白表达向量。证实了噬菌体的合成蛋白质蛋白质电泳和杆状结构由电子显微镜进行了分析。发现:我们的工作都集中在开发另一种抗原决定基呈现杆状平台可用于生物医学应用。 To our knowledge, it is the first attempt to produce bacteriophage originated nanotubes in yeast cells which determines their tolerance for genetically incorporated foreign epitopes. Yeast protein synthesis system allowed efficient generation of long and flexible nanotubes originated from NBD2 tailed bacteriophage as well as tubes with different morphology from RaK2 and FV3 phages. Conclusion & Significance: This work intends to show the suitability of yeast protein synthesis system to generate high yields of nanotubes that originate from tailed bacteriophages. The novel strategy presented here could provide safer vaccine candidates compared to the VLPs synthesized in bacteria. Recent Publications 1. Kaliniene L, Truncait?? L, ?imoli?nas E, Zajan?kauskait?? A, Vilkaityt?? M, Kaupinis A, Skapas M and Me?kys R (2018) Molecular analysis of the low-temperature Escherichia coli phage vB_EcoS_NBD2. Archives of Virology 163(1):105-114. 2. Schoonen L and van Hest J C (2014) Functionalization of protein-based nanocages for drug delivery applications. Nanoscale 6(13):7124-41. 3. Zeltins A (2013) Construction and characterization of virus-like particles: a review. Mol Biotechnol. 53(1):92-107. 4. Jennings G T and Bachmann M F (2008) The coming of age of virus-like particle vaccines. Biol Chem. 389(5):521-36. 5. Trivedi B, Valerio C and Slater J E (2003) Endotoxin content of standardized allergen vaccines. J Allergy Clin Immunol. 111(4):777-83.
