All submissions of the EM system will be redirected toOnline Manuscript Submission System. Authors are requested to submit articles directly toOnline Manuscript Submission Systemof respective journal.

Abstract

Study on the anti-quorum sensing activity of a marine bacterium Staphylococcus saprophyticus 108

Author(s):Miao Li, Zhou Huiru, Ding Biting, Jiang Yun, Jiang Wei, Dong Kunming

Quorum sensing inhibitors (QSI) may provide new research direction for the defense of bacterial diseases and biofilm related marine biofouling. QSI screening and development from natural source have received great interest in recent years. In this study, 17 marine bacterial strains, which were found exhibiting anti-quorum sensing activity in previous study, were secondary screened using pigment inhibition assay. One active bacterium 108 was identified as Staphylococcus saprophyticus based on the 16S rDNA sequence analysis and morphological observation. The culture condition of bacterium 108 was optimized. The results showed that moderate pH (6.0-7.0) and temperature (25°C) favored the bacterial growth and bioactive metabolites production. Yeast extracts content was the most importantmediumcomponent affecting the bacterial growth and bioactive metabolites production. By culturing under the optimized condition, the anti-quorum sensing activity of S. saprophyticus 108 and the yield of crude extracts was increased about 50% and 1.5 times, respectively, compared to the bacterial cultures before optimization. The active metabolites in S. saprophyticus 108 cultures were further extracted, isolated and identified. Cyclo (Pro-Leu), was identified from the active subfraction of the bacterium by GC-MS analysis. It was found for first time that Cyclo (Pro-Leu) exhibitedmoderate anti-quorumsensing activity.


Share this

Select your language of interest to view the total content in your interested language

Table of Contents

Google Scholar citation report
Citations : 875

BioTechnology: An Indian Journal received 875 citations as per Google Scholar report

Indexed In

  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Cosmos IF
  • 直接ory of Research Journal Indexing (DRJI)
  • Secret Search Engine Labs
  • Euro Pub

Read More

Global Tech Summit