Abstract

Stability-indicatingmethods for determination of terazosin in presence of its degradation product

Author(s):MoatazA.Yehia, Mamdouh R.Rezk, MohamedA.El-Sayed, MohamedAbdel Kawy

Four different stability indicating assay methods were developed and validated for the determination of terazosin in the presence of its degradation product. The first and second method was based on the derivative and derivative ratio spectrophotometric technique using methanol as a solvent. In the third method, we used TLC-densitometric technique using high performance thin-layer chromatography plates with a developing system consisting of chloroform: acetone: ammonia (6:4:0.1, by volumes). The fourth method was a high performance liquid chromatography. Separation of terazosinfrom itsdegradate using C18 column and a mobile phase consisting of acetonitrile: methanol: water: tri-ethyl amine (v/v pH 5.6), in the ratio of (45: 45: 10: 0.2, by volume) at ambient temperature was achieved. The developed methods were successfully applied to the analysis of pharmaceutical formulations containing terazosinwith excellent recoveries.