摘要

反相高效液相色谱法同时测定人血浆中磺胺甲恶唑和甲氧苄啶的含量

作者(年代):Syed N.Alvi, Muhammad M.Hammami

建立了一种简便、精确的反相高效液相色谱法同时测定人血浆中磺胺甲恶唑(SUZ)和甲氧苄氨嘧啶(TMP)的方法，并进行了验证。以头孢美唑钠为内标(IS)，在Symmetry Shield C18 (4.6ï´150 mm, 4 ï . m)色谱柱上进行分离。流动相为30mm磷酸钠(pH 5.8)、乙腈和0.05%三乙胺(83:17:0.05，)v/v，流速为1.2ml/min。用235 nm分光光度法检测洗脱液。血浆样品用乙腈沉淀，提取液蒸发后在磷酸钠缓冲液中重建。空白血浆和常用药物均无干扰。TMP和SUZ浓度与其与IS的峰高比值在0.10-6.0和1.0-70 ï g/ ml范围内呈线性关系。TMP和SUZ的日内和日间变异系数分别为ï‑5.2%和8.7%，ï‑7.0‑11.3%。从血浆样品中提取TMP、SUZ和IS的回收率分别为95%、85%和80%。该方法用于评估TMP和SUZ在临床实验室通常遇到的各种情况下的稳定性。 TMP and SUZ in plasma were stable for at least 24 hr at RT, 8 weeks at -20ºC; and after three freeze-thaw cycles. TMP and SUZ in processed samples were stable at least 24 hr at RT, and 48 hr. at -20ºC. Stock solutions of TMP and SUZ in mobile phase were stable at 24 hr at RT, 8 weeks at -20ºC.