摘要

罗非鱼幼鱼消化和代谢酶对膳食单宁的反应

作者(年代):阿希萨·普鲁斯蒂，s·莫哈帕特拉，p·比斯瓦斯，n·p·萨胡，a·k·帕尔

为研究饲粮单宁对罗非鱼(Labeo rohita)幼鱼消化代谢酶反应的影响，进行了为期60 d的饲粮试验。试验选用平均体重3.2g的育苗180尾，随机分为6个处理，每个处理3个重复。分别配制6种等氮(粗蛋白质33.98 ~ 35.11%)、等热量(394.42 ~ 394.74 Kcal DE/100g)饲粮，各组单宁酸水平分别为对照(0%)、T1(0.25%)、T2(0.5%)、T3(1.0%)、T4(1.5%)和T5(2.0%)。蛋白酶活性与饲粮单宁浓度呈负相关。饲粮添加单宁酸显著(P<0.05)抑制了肠道淀粉酶活性。脂肪酶活性与膳食单宁含量呈负相关。单宁酸组肝脏糖异生酶G6Pase和FDPase活性显著(P<0.05)低于对照组。FDPase活性与饲粮单宁含量呈负相关。肝脏中G6PDH活性在较低浓度下显著升高(P<0.05)，在0.5%单宁时最高。较高浓度单宁酸(T4和T5)显著提高了肌肉AST和ALT活性(P<0.05)。 Muscle ATPase activity showed decrease in its activity with increase in tannic acid in the diet. From this experiment it seems that dietary tannin even at 0.25% level, significantly affects the digestive and metabolic enzyme of L. rohita fingerlings. This may be considered by the nutritionists while formulating diet for this species.