摘要

黑籽对缺血-再灌注心肌损伤的保护作用

作者(年代):苏拉·穆罕默德·卡迪姆

心肌梗死损伤的缺血-再灌注(I/R)模型可能发生在各种临床环境中，这仍然是一个重要的问题。再灌注时产生的氧自由基已被证明在I/R损伤中起主要作用。各种治疗效果已被描述为黑草。此外，还发现黑草对各种器官缺血再灌注损伤具有保护作用。因此，黑草可能对心脏缺血再灌注损伤有保护作用。本研究旨在评价黑籽对心肌梗死(MI)缺血-再灌注(I/R)模型的心脏保护作用。将36只大鼠分为3组，分别为对照组(1组)、I/R组(2组)和黑草治疗组(3组)。本实验选用体重200 ~ 230 g的雄性Wistar大鼠36只。所有动物都在标准条件下饲养。大鼠术前24小时不进食，不饮水。将大鼠分为假手术组(第1组)、缺血(45 min)-再灌注(60 min) I/R组(第2组)和黑草油处理组(第3组)。所有大鼠均以40 ~ 50 mg/Kg硫喷妥钠麻醉。 Nigella sativa oil was given to the rats in treatment group, before ischemia and before reperfusion at a dose of 0.2 mL/Kg by intraperitoneal route. We chose the dose of this agent according to reported studies about I/R and Nigella sativa oil, as this dose has been shown to be effective in previous studies 14,15 . Rats in the I/R group were infused only with saline. The study showed that the Nigella sativa (orientalis) oil contain: glycosides, saponins, tannins, phenolic compounds, resins, alkaloids, proteins and flavonoids. The levels of GSH, SOD, GSHPx, CAT enzymes in treatment group were significantly higher than those in the group I/R, but MDA in treatment group were lower than in the I/R group. Our results suggest that Nigella sativa (black seed) treatment protects the rat heart against ischemia-reperfusion model of myocardial infarction (MI).