文摘
甲基乙二醛改性DNA:可能引发糖尿病的病理生理学
作者(年代):Sana阿拉姆,Aditya Verma Akif Ahsan, Sarita阿加瓦尔背景:甲基乙二醛(毫克),高活性亲电试剂,是glycating剂有更高的反应性比葡萄糖。先前的研究已经表明,MG与proteinsa反应氨基酸组,形成先进的糖化终端产品和自由氨基的核酸的形成导致DNAa年龄。先前的研究已经完成显示MG的浓度及其派生的年龄增加患者糖尿病及其并发症。至今不过,大多数研究都集中在葡萄糖和蛋白质之间的糖化反应,葡萄糖是最丰富的糖在血液和大幅升高糖尿病。研究的目的我们的目的是分析MG对DNA的影响和评估的影响糖化DNA在2型糖尿病的发病机理。主题和方法:在这项研究中,DNA被修改的糖化反应与赖氨酸MG Cu2 +的存在。修改后的DNA已经被各种生化的技术特点,如(UV / Vis)吸收光谱和荧光光谱分析。MG-modified-DNA表现出广泛的损害所揭示的各种物理化学研究。抗体分析由直接绑定和竞争性抑制ELISA。MG-modified-DNA显示增色性增加,荧光强度增加。 Sera of diabetic patients showed preference for MG-modified-DNA in comparison to native DNA. Inhibition ELISA supported the above findings. MG-modified-DNA emerged as a powerful inhibitor as compared to native DNA. Results: Our study showed that the structural perturbations in MGâLysâCu2+-DNA which implied the generation of new epitopes that might have made the molecule immunogenic and furthermore, it suggested that DNA glycated with MG may trigger an immune response which might result in generation of anti-glycated DNA antibodies (sera autoantibodies) which are preferably bound by glycated-DNA as compared to native DNA in Type 2 DM patients.
