文摘
测量肝灌流液的氧化DNA损伤水平与铜溶液预处理后用高效液相色谱电化学检测
作者(年代):Csilla MiA一个¾方差分析¡,安东科比本研究的目的是开发一个rp方法测定背景8-oxodG(水平生物标志物氧化应激)在大鼠肝脏灌流液添加铜。反相分析柱PurospheriA一STARC18e (150 x4.6mm,身份证5 iAm,默克公司)与PurospheriA明星RP-18e (4 x4mm,身份证,5 ïÂm,Merck) as a precolumn were applied for the analysis. The mobile phase consisted of 8% (v/v) methanol in 50mmol/Lphosphate buffer, pH5.5.Oxidative damage to nuclear DNAwas determined by the simultaneousmeasuring of 2ÂÂ- deoxyguanosine (dG) with UV detection folowed by electrochemical detection of 8-oxodG. The validation of the HPLC method according to linearity, accuracy and precision was investigated. A detailed investigation of experiments have been discussed in this paper.
