文摘

非生物和生物因素对发育生物学的影响四种蕨类植物的西高止山脉,印度

作者(年代):M。约翰逊,V.S.Manickam

目前研究了年龄的影响,季节和pH值对孢子萌发,配子体早期发育和四个珍稀濒危蕨类植物的孢子体的形成,即Cheilanthes viridis (Forssk)。斯沃茨,Phlebodium aureum L。,Pronephrium triphyllum (Sw)。Holttum和Sphaerostephanos unitus (l) (Holttum)高止山脉西部的南印度。比例最高的孢子萌发实现只有在成熟孢子接种媒体,而年轻的孢子发芽。达到了最高比例的孢子萌发孢子收集在2月的月Cheilanthes冬青;7月——Phlebodium aureum;March-Pronephrium triphyllum和1月Sphaerostephanos unitus。孢子萌发的发生在一个广泛的pH值从4到8。Cheilanthes viridis,孢子萌发的比例最高(88.8 ia‚±1.61)发生在努森C Solidmediumat pH值5.8。 Mitra liquid medium at pH 5.5 showed highest percentage (81.30.84) of spore germination for Phlebodium aureum. Pronephrium triphyllum (38.31.21) and Sphaerostephanos unitus (36.81.31) showed highest percentage of spore germination on Knops solid medium at pH 5.8. There were nomorphological differences among gametophytes grown under different pH optima. Gametophytes gave rise to new gametophytes and developed antheridia and archegonia after they were transferred to culture flasks. In Cheilanthes viridis (Forssk.) Swartz, high growth rate as well as high percentage (85.81.15) of sporeling formation were observed in KC mediumat pH5.8. In Phlebodium aureumL. highest growth area aswell as highest sporeling emergence were recorded (33.41.21) inMi medium at pH 5.5. In Pronephriumtriphyllum (Sw.) Holttum, highest growth area and highest percentage (52.31.21) of sporeling emergence were observed in KNmediumat pH 5.8. In Sphaerostephanos unitus (L.) Holttum, KNmedium at pH 5.8 induced a maximum growth rate and high percentage (76.81.21) of sporeling emergence. The Isozyme profiles revealed the biochemical changes between the different developmental stages of selected ferns.