摘要

非生物和生物因素对发育生物学的影响可能是印度西部高山岭四种蕨类植物濒危的原因

作者(年代):M.Johnson, V.S.Manickam

本文研究了4种珍稀濒危蕨类植物雪兰(Cheilanthes viridis, Forssk.)的年龄、季节和pH值对孢子萌发、早期配子体发育和孢子体形成的影响。斯瓦茨，金黄色黄脉，三叶原脉(西南)南印度西部高止山脉的Holttum和Sphaerostephanos unitus(L.)(Holttum)。仅在成熟孢子接种培养基中萌发率最高，而年轻孢子未萌发。2月份采集的雪兰孢子萌发率最高;七月金静脉;3月-三叶前叶和1月为雪粉。在pH值为4 ~ 8的范围内，孢子都能萌发。在pH为5.8的Knudson C固体培养基上，雪兰孢子萌发率最高(88.8±1.61)。pH为5.5的Mitra液体培养基孢子萌发率最高(81.3±0.84)。 Pronephrium triphyllum(38.3±1.21) and Sphaerostephanos unitus(36.8±1.31) showed highest percentage of spore germination on Knops solid mediumat pH 5.8. There were no morphological differences among gametophytes grown under different pH optima. Gametophytes gave rise to new gametophytes and developed antheridia and archegonia after they were transferred to culture flasks. In Cheilanthes viridis(Forssk.) Swartz, high growth rate as well as high percentage( 85.8±1.15) of sporeling formation were observed in KC medium at pH 5.8. In Phlebodium aureum L. highest growth area as well as highest sporeling emergencewere recorded(33.4±1.21) in Mi medium at pH 5.5. In Pronephrium triphyllum(Sw.) Holttum, highest growth area and highest percentage(52.3±1.21) of sporeling emergence were observed in KN medium at pH 5.8. In Sphaerostephanos unitus(L.) Holttum, KN medium at pH 5.8 induced amaximumgrowth rate and high percentage(76.8±1.21) of sporeling emergence. The Isozyme profiles revealed the biochemical changes between the different developmental stages of selected ferns.