摘要

龙葵总粗提物对Wistar白化大鼠的免疫调节作用

作者(年代):Aloysius M.B.Lubega, Godfrey S.Bbosa, Nathan Musisi, Joseph Erume, Jasper Ogwal-Okeng

免疫系统是人体的防御机制，目前世界范围内的许多疾病都在加剧或抑制免疫系统。本研究探讨了金针叶总粗提物对白化病大鼠的免疫调节作用。采用4组，每组6只体重在80 ~ 150g之间的健康雄性成年动物。第一组大鼠ig生理盐水1mL。II组、III组和IV组每日分别给予总粗提物1mL，剂量为125mg/Kg bwt、250mg/Kg bwt和500mg/Kg bwt，连续14 d。第15天，穿刺大鼠眶后静脉采集全血。测定红细胞、免疫血细胞计数、血凝素滴度、甲状旁腺激素。将提取物的剂量从125毫克/公斤体重增加到500毫克/公斤体重，导致平均白细胞计数减少。与对照组相比，500mg/Kg体重的减少具有统计学意义(pïÃ， Â， 0.05英镑)。在所有剂量下，平均RBC计数均高于对照组，但无统计学意义(pïÃ， Â，³0.05)。 Themean%lymphocyteswere higher for all the doses than the control group but with no statistical difference between the 2 groups (p0.05) though however, there was a dose-dependent reduction in the mean%lymphocytes. The mean%lymphocytes were higher for all the doses than the control group but with no statistical difference between the 2 groups (p0.05) though however, there was a dose-dependent reduction in the mean % lymphocytes. The HA titer was higher than the control group and was statistically significant (pï‚£0.05) at 125 and 250mg/ Kg bwt as compared to the control group. The neutrophil adhesion were higher than the control group but were not statistically significant. The DHT were higher than for the control group and was statistically significant (p0.05) at a dose of 500mg/Kg bwt after 12 hours and 125mg/Kg bwt after 24 hours. The results show that the total crude leaf extracts of S. anguivi contained compounds with immunomodulatory activity.