摘要
香蕉皮和沙粒固态发酵下聚乙烯修饰脂肪酶的经济生产
作者(年代):玛格达·a·el - bendary，梅萨·e·莫哈拉姆，多娅·a·r·马哈茂德以农业Â -工业副产物残渣为底物，以沙粒为载体，在分离芽孢杆菌固态发酵条件下生产聚乙烯表面修饰脂肪酶。香蕉皮的酶产率最高，为4.5- 6%(732U/g发酵培养基)。在初始含水率为10%，孵育温度为30ï‑1°时，脂肪酶产量最高。以醋酸铵为碳源富集培养基，酶产率提高31%左右。另一方面，氮源蛋白胨使酶产率提高了28%。最佳初始pH值为7 d，潜伏期为5 d。1%氯化钙和氯化钾的酶产率分别提高了12%和9%。在培养基中添加表面活性剂对酶产率无影响。在最佳ssf条件下，在铝槽中进行了脂肪酶的中试生产。发酵后脂肪酶活性为1397 U/g。 This result was comparable with that obtained frombench scale production (in flasks). The cost of one kilogram of this fermented culture containing 1397000 U of lipase was estimated as US $ 2.4. The enzyme showed the highest activity at 70ï°C and pH 8.5. It was stable up to 50ï°C for 15 min and pH range 5-9 for one hour. The obtained results were discussed in the light of possible utilization of banana peels and sand for the production of this important industrial enzyme under solid state fermentation.
