文摘
不同光度法同时测定的andRP-HPLCmethods aceclofenac和扑热息痛片剂剂型
作者(年代):七弦琴Poonam Prakash帕蒂尔,Smita Sampatrao户伸,桑杰Kasture一个简单的区别spectrophotometricmethod了aceclofenac估计和扑热息痛frompharmaceutical剂型。pH值的变化改变了吸收光谱aceclofenac和扑热息痛。微分spectrumof扑热息痛和aceclofenac在碱性和酸性溶液showedmaximumabsorbance 267 nmand 246海里和最小值分别为232 nm和276 nm。测量吸光度进行了零交点波长247 nmfor aceclofenac扑热息痛和256.7海里。比拉年代法律有效浓度范围的1-20ig / ml aceclofenac和1-50µg /毫升扑热息痛。这个方法被验证精度(n = 6)和准确性(100.15我±0.761%)和(99.73我±0.436%)分别在每个级别(n = 3)。反相高效液相色谱(rp),开发和验证的同时估计扑热息痛(PARA)和aceclofenac (ACE)片剂剂型。WatersC18十八基硅烷(ODS)列(250我´4.6 mmi。d。5我在isocraticmodewas粒度)使用。使用的流动相乙腈:50 mM磷酸二氢钠(50:50)v / v, pH3.0±0.1正磷酸。 The flow rate was 1.5 mL per minute and eluent was monitored at 276 nm. The retention times of PARAand ACE were found to be 1.92 min and 9.28 min respectively. The linearity for paracetamol and aceclofenac were in the range of 5 - 50ïg /mL and 1.0 - 10ïg /mL respectively (n=6). Percentage recoveries obtained for paracetamol and aceclofenac were 100.7% and 100.4 % respectively. The relative standard deviationwas found to be 1.78%and 1.80%for paracetamol and aceclofenac in triplicate analysis. Thus the proposed methods are precise, accurate, selective and rapid for simultaneous estimation of paracetamol and aceclofenac in tablet formulation.
