文摘

2018年生物化学:转录调节细胞周期蛋白D1表达式YB-1和NKX2-1人类非小细胞肺癌

作者(年代):正敏北川

已知的致癌基因细胞周期蛋白D1 (CCND1)参与的发展细胞周期从G1 S期。表达的细胞周期蛋白D1经常在多种人类癌症包括提升非小细胞肺癌症(NSCLC)。转录因子YB-1和NKX2-1(也称为TTF-1)经常表现在非小细胞肺癌。我们发现Y-box和NKX2-1绑定周期蛋白D1的启动子序列。然而,细胞周期蛋白D1的功能协会YB-1和NKX2-1尚未完全阐明。在研究中,我们表明,不仅YB-1 NKX2-1促进了细胞周期蛋白D1的转录。此外,芯片分析表明,他们一定会绑定周期蛋白D1的启动子序列。有趣的是,额外的表达YB-1增强NKX2-1-mediated启动子活性的细胞周期蛋白D1。因此,YB-1 NKX2-1可能协同促进细胞周期蛋白D1的转录。此外,我们发现,细胞周期蛋白D1的表达明显与YB-1以及NKX2-1人类非小细胞肺癌临床标本。这些结果强烈表明YB-1和NKX2-1是细胞周期蛋白D1的转录因子表达在人类非小细胞肺癌。 Overexpression of cyclin D1 has been found in NSCLC. It has been reported that overexpression of cyclin D1 is not only positively, but also negatively correlated with poor prognoses. Therefore, the association between cyclin D1 expression and prognosis is controversial. Here, we found that cyclin D1 expression was significantly correlated with NKX2-1 in human adenocarcinomas but not squamous cell carcinomas. NKX2-1 was a good prognosis factor in cyclin D1-positive adenocarcinomas. Expression of NKX2-1 but not cyclin D1 was inversely associated with metastatic incidence as an independent good prognostic factor of adenocarcinoma. Altogether, NKX2-1-expressing adenocarcinomas, whereas NKX2-1 promoted cyclin D1 expression, may show good prognosis features by the metastasis inhibition potency of NKX2-1 regardless cyclin D1 expression.