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橘皮苷的保护作用与基因毒性的评估和生化环磷酰胺对小鼠的影响。

作者(年代):一个。Abd El Raouf克里Girgis, H.S.Abdou,埃曼M.Saleh

橘皮苷(淬火)是一种自然产生的类黄酮素广泛存在于柑橘类水果。Flavonoidswere inhibitDNAand染色体环磷酰胺诱导的损害。化学预防是一种最有前途的和现实的方法预防环磷酰胺治疗产生的副作用的抗癌药物。因此,本研究旨在评估淬火对基因毒性的保护作用和生化的影响环磷酰胺(抗肿瘤药)在瑞士白化病雄性老鼠。老鼠被分为19组(5每个动物)。1 stgp担任控制。第二,3 rd,四、gps分为9组,收到口头50,100和200毫克/公斤体重的环磷酰胺,分别为2和3周。5日,6日,7日gps分为9组,收到50,100和200毫克/公斤体重的环磷酰胺结合50毫克ofHDNfor 1、2和3周,分别。最后的实验时间,动物受到遗传和生化分析。结果显示thatMNfrequency显著增加环磷酰胺处理的小鼠。然而,淬火处理显示显著decreament MN频率inmice bonemarrowcells相比控制。Aswell, a significant inhibition in DNA content using comet assay in cytoxan treated groups with 3 fold lower than control,whereas the treatmentwithHDN in improved that content. Also, there was a significant reduction in DNA, RNA and protein content in brain and kidney tissues and functions (creatinine and urea level elevation).However, administration of HDNin combinationwith cytoxan significantly improved all parameters studied in brain and kidney tissues.Asignificant decrease in the level of tissue antioxidants like superoxide dismutase (SOD), cholinesterase (CHE) and increase in malondialdhyde (MDA), cholesterol, glucose and triglycerollevelswas found in cytoxan treated groups. In conclusion, HDN supplementation significantly ameliorate these parameters due to antioxidant activity, thereby showing potent antigenotoxic and chemopreventive effects against toxicity induced by cytoxan in mice.