摘要
利用自制的FIA/合并区技术,建立了分光光度法测定不同产地药物样品中色氨酸的新方法
作者(年代):BushraB。卡西姆,萨拉·f·哈米德提出了一种快速、灵敏的间歇式流动注射—合并区分光光度法测定纯物质和制剂中的色氨酸。该方法是将试剂二苯胺磺酸盐(DASA)在硫酸介质中与亚硝酸钠反应后氧化为二苯联苯胺磺酸。不稳定氧化产物与亚硝酸钠迅速反应产生重氮化中间体。重氮化中间体与色氨酸偶联。开发了一种在300C下稳定1 h的粉红色产品。上色产物在522nm处测量。研究了化学反应物的最佳浓度和物理仪器条件。峰高与浓度的线性图表明,在3-40和5.100µg的浓度范围内,BeerÂ′s的低是服从的。色氨酸的检出限为0.2325和0.188µg。ml- 1的色氨酸分别用于批次和FIA方法。优化后的FIA体系能够测定色氨酸。 with a through put 52 samples.h-1. The proposed methods were applied successfully for the determination of Tryptophan in pharmaceutical preparations and statistical analysis of the results were compared with results by the British pharmacopeia were also reported.
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